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Immuno diagnostics > Immunofluorescence specific IgE assay kit
Immunofluorescence specific IgE assay kit
Intended use:

Immunofluorescence specific IgE assay is used for screening the specific antibody (IgE) that recognizes some common allergens.

Introduction:

Allergic or immediate hypersensitivity reactions are known as Type I immunopathologic reactions that may occur within minutes of an allergic challenge. The most basic allergy symptoms are similar to those of common cold-snuffling, itchy eyes, and sneezing. Allergy can also cause skin rashes, hives or weal such as contact dermatitis or eczema.

In 1967 Ishizaka identified the serum factor capable of mediating allergic reactivity as Immunoglobulin E (IgE). Immunofluorescence specific IgE assay is based on the principle of Fluorescent immunoassay, a rapid test where an antibody or antigen is linked to a fluorescent as a means of detecting a match between the antibody and antigen. It is used to identify in your blood the existence of specific antibody (IgE) that recognizes some common allergens .

Principle of the assay:

Allergen (recombinant protein or mixture of specifically purified protein) is spotted onto the surface of the reaction well. Such allergen can bind to the specific IgE in the serum sample that can further recognize Cy3 attached secondary antibody. Signal intensities of the Cy3 are then measured and analyzed using high efficiency laser scanner. The results obtained can then used to calculate the specific IgE concentration of the serum sample.



Figure:

Immunofluorescence specific IgE assay kit is developed by Excelsior Bio-System Inc. and produced by Formosa biomedical technology corp., cooperatively.
Excelsior Bio-System: www.excelsior-biosystem.com.tw .


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