Immunofluorescence specific
IgE assay is used for screening
the specific antibody (IgE) that
recognizes some common allergens.
Introduction:
Allergic or immediate hypersensitivity
reactions are known as Type I
immunopathologic reactions that
may occur within minutes of an
allergic challenge. The most basic
allergy symptoms are similar to
those of common cold-snuffling,
itchy eyes, and sneezing. Allergy
can also cause skin rashes, hives
or weal such as contact dermatitis
or eczema.
In 1967 Ishizaka identified the
serum factor capable of mediating
allergic reactivity as Immunoglobulin
E (IgE). Immunofluorescence specific
IgE assay is based on the principle
of Fluorescent immunoassay, a
rapid test where an antibody or
antigen is linked to a fluorescent
as a means of detecting a match
between the antibody and antigen.
It is used to identify in your
blood the existence of specific
antibody (IgE) that recognizes
some common allergens .
Principle
of the assay:
Allergen (recombinant protein
or mixture of specifically purified
protein) is spotted onto the surface
of the reaction well. Such allergen
can bind to the specific IgE in
the serum sample that can further
recognize Cy3 attached secondary
antibody. Signal intensities of
the Cy3 are then measured and
analyzed using high efficiency
laser scanner. The results obtained
can then used to calculate the
specific IgE concentration of
the serum sample.
Figure:
Immunofluorescence specific IgE
assay kit is developed by Excelsior
Bio-System Inc. and produced by
Formosa biomedical technology
corp., cooperatively.
Excelsior Bio-System: www.excelsior-biosystem.com.tw
.
